Extended Data Fig. 7. Glycolytic metabolism enhances cell survival and cell proliferation.

Extended Data Figure 7 (related to Fig. 3). a, Apoptosis assay in HSC2 cells pretreated with doxycycline for at least 4 days. Propidium iodide (PI) and annexin V double positive cells were considered as dead cells. Data indicate mean ± S.D. b, Cell proliferation assay in SCC13 cells pretreated with doxycycline for at least 3 days with or without DCA over time. Data indicate mean ± S.E.M. (left). Western blot analysis of whole cell lysates was performed at day 5 in the cell proliferation assay (right). c, Schematic presentation of skin xenotransplantation assay in severely immunocompromised NSG (NOD/SCID/Il2rg^−/−) mice. Doxycycline was administered in drinking water. HPKs; human primary keratinocytes, TAFs; tumor-associated fibroblasts d, In vivo growth of SCC13 cells was monitored by bioluminescence imaging over time. Normalized total flux ratio of each mouse was used to track in vivo tumor growth (left). Representative bioluminescence images of tumors with control or SIRT6-deficient SCC13 cells at day 61 (right). e, Knockdown of SIRT6 by in vivo doxycycline administration in tumors was confirmed by immunohistochemistry. Scale bars indicate 100μm. Statistics, sample sizes (n) and numbers of replications are presented in Methods, ‘Statistics and reproducibility’. * p<0.05, *** p<0.001