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. Author manuscript; available in PMC: 2021 Aug 22.
Published in final edited form as: Nat Metab. 2021 Feb 22;3(2):182–195. doi: 10.1038/s42255-021-00350-6

Extended Data Fig. 3. Metabolic features of tumor basal cells.

Extended Data Fig. 3

Extended Data Figure 3 (related to Fig. 2). a, GLUT1 and Keratin5 (left panel), and GLUT1 and Keratin10 (right panel) immunofluorescence in skin tumor samples treated with DMBA/TPA. Scale bars indicate 200μm. Data indicate mean ± S.E.M. b, CD34 and Keratin5 (left panel), and CD34 and Keratin10 (right panel) immunofluorescence in skin tumor samples treated with DMBA/TPA. Scale bars indicate 200μm. Data indicate mean ± S.E.M. c, Representative whole tumor immunofluorescence with GLUT1, CD34, and Keratin 10 in skin tumor samples treated with DMBA/TPA. Images were taken at 40x and stitched with the software in Zeiss confocal microscope. Correlation value is calculated in Matlab. Scale bars indicate 300μm. d, Representative flow cytometry plot of GLUT1-A647 and CD34-BV421 co-stained tumor cells after gating live, YFP positive, a6 integrin high cells. Statistics, sample sizes (n) and numbers of replications are presented in Methods, ‘Statistics and reproducibility’.