Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 8;15:997–1011. doi: 10.2147/DDDT.S288369

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Ubieta et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

Increased Th17-like polarized CD4+ T-cell number after pretreatment with nintedanib. Flow cytometry analyses of CD4+ T cells in CD3+ T-cell culture from healthy donors pretreated for 20 min with or without nintedanib at 10, 30 and 100 nM and stimulated for 24 hours with αCD3/αCD28, αIL-4, αIFN-γ and IL-2. (A) FACS dot plots show one representative experiment of CD4+ gated, IL-17+ cells. (B) Percentage and (C) absolute counts of the total CD4+/IL-17+ T cells in total CD3+ T-cell culture (n=4–6). (D) IL-17A in the cell culture supernatant was determined by ELISA. Bars represent mean values ± standard deviation, *P<0.05.

Abbreviations: ELISA, enzyme-linked immunosorbent assay; FACS, fluorescence-activated cell sorting; IFN-γ, interferon gamma; IL, interleukin; SSC, side-scatter.