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. 2021 Mar 8;15:997–1011. doi: 10.2147/DDDT.S288369

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© 2021 Ubieta et al.

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Excess IL-4 and IFN-γ prevent the increase of Th17-like cells induced by nintedanib. Flow cytometry analyses of CD4+ T cells in CD3+ T-cell culture from healthy donors pretreated for 20 min with or without nintedanib at 10, 30 and 100 nM and stimulated for 24 hours with αCD3/αCD28, rhIL-4, with (rescued) or without (unrescued) rhIFN-γ and IL-2. (A) FACS dot plots show one representative experiment of CD4+ gated, IL-17+ cells. (B) Percentage and (C) total number of CD4+IL-17+ T cells in total CD3+ T-cell culture (n=4-6). Bars represent mean values ± standard deviation, *P<0.05. + = unrescued, R = rescued.

Abbreviations: FACS, fluorescence-activated cell sorting; IFN-γ, interferon gamma; IL, interleukin; rh, recombinant human; SSC, side-scatter.