Figure 7.
Enhanced cellular recycling and half-life of ABD-IgA1 fusion in the presence of engineered albumin. A) IgA1ABD variants were pre-incubated with QMP or WT albumin, and then given to HMEC-1 cells overexpressing human FcRn. The data is presented as relative recycling where IgA1 is set to 1.0. The numbers given represent duplicates in ELISA of triplicates in HERA (SD). ns > 0.05, **** = p < .0001, by two-tailed analysis using unpaired T-test (df = 10, t = 2.99, 11.05). B) Log-linear changes in plasma level (%) of IgA1-LCABD when given together with WT or QMP, and, IgA1-HCABD when given together with WT or QMP, in human FcRn transgenic mice lacking expression of mouse albumin. The variants were given by i.v. injections to 5 mice per group. ns > 0.05, * = p 0.0342, by two-tailed analysis using unpaired T-test (df = 8, t = 2.23, 2.55)