Table 1.

Apparent IC₅₀ values of known irreversible inhibitors against p97 and its reporters

Compounds	Structures	Apparent IC50 (μM)a
		UbG76V-GFP	ODD-Luc	p97
1b (JFD02342)		3.4 ± 1.0	5.2 ± 1.8	0.2 ± 0.02
2b (β-nitrostyrene)		1.6 ± 0.4	3.9 ± 1.0	1.7 ± 0.5
3 (EerI)		3.7 ± 0.4	NDc	> 30
4 (cmk)		5.8 ± 1.1	4 ± 2	3.9 ± 0.9
5 (fmk)		47 ± 10	39 ± 3	> 30
6		18 ± 4	11 ± 5	10 ± 5

^aMeasurements were carried out in triplicate, and variance is expressed as the standard deviation.

^bData for compounds 1 and 2 were adapted from references [21] and [41], respectively.

^cND: Not Determined