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. Author manuscript; available in PMC: 2022 Mar 5.
Published in final edited form as: Eur J Med Chem. 2021 Jan 2;213:113148. doi: 10.1016/j.ejmech.2020.113148

Table 3.

Apparent IC50 values of the pyrazolo[3,4-d]pyrimidine analogs against p97, T532C-Cdc48, and the cellular reporters.

Compounds Apparent IC50 (μM)*
UbG76V-GFP ODD-Luc p97 T532C-Cdc48
17 > 30 > 30 > 100 > 100
18 (PPA) 1.6 ± 0.5 35 ± 10 0.6 ± 0.2 14 ± 5
19 5.4 ± 1.6 > 30 1.1 ± 0.7 26 ± 6
20 31 ± 3 > 30 4.5 ± 3.5 35 ± 8
21 > 30 > 30 > 100 > 100
22 2.0 ± 0.9 > 30 3 ± 1 24 ± 7
23 2.5 ± 0.3 > 30 1.3 ± 0.7 22 ± 11
24 3.6 ± 0.5 > 30 10 ± 6 ND
25 > 30 > 30 > 50 ND
26 > 30 > 30 > 50 ND
27 2.1 ± 0.6 > 30 2.8 ± 1.2 ND
28 6.1 ± 0.7 > 30 0.9 ± 0.2 ND
*

Measurements were carried out in triplicate, and variance is expressed as the standard deviation. ND: not determined.