Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | Lphn2 cKO | PMID:28972101 | JAX ID 023401 | |
| Genetic reagent (Mus musculus) |
HA-Lphn3 cKO | PMID:30792275 | JAX ID 026684 | |
| Genetic reagent (Mus musculus) | CD-1 | Charles River Laboratory | Pure CD-1 background mice used to generate primary hippocampal cultures for surface expression experiments. | |
| Cell line (Homo sapiens) | HEK293T | ATCC | CRL-11268 | Mycoplasma testing was not performed since cells were maintained for a maximum of 10 passages. |
| Cell line (Homo sapiens) | HEK293F | Thermo Fisher | R79007 | Mycoplasma testing was not performed since cells were maintained for a maximum of 10 passages. |
| Recombinant DNA reagent | Lentiviral Syn NLS-GFP-Δcre | PMID:21241895 | Also used in PMID:30792275 | |
| Recombinant DNA reagent | Lentiviral Syn NLS-GFP-CRE | PMID:21241895 | Also used in PMID:30792275 | |
| Recombinant DNA reagent | pCMV WT hLphn3 | PMID:30792275, 26235030 | N-terminal IgK signal peptide followed by FLAG | |
| Recombinant DNA reagent | pCMV hFLRT3 | PMID:30792275, 26235030 | N-terminal preprotrypsin signal peptide followed by MYC | |
| Recombinant DNA reagent | pcDNA hTen2 | PMID:30792275, 26235030 | C-terminal FLAG; extracellular HA prior to transmembrane region | |
| Recombinant DNA reagent | pCAG WT rLphn2 | PMID:28972101, 30792275 | N-terminal preprotrypsin signal peptide followed by FLAG; intracellular mVenus | |
| Recombinant DNA reagent | pCMV Lphn3-ECD | This paper | hLphn3 residues 20–923 with an N-terminal NCAM1 signal peptide and a C-terminal NCAM1 GPI-anchoring sequence | |
| Recombinant DNA reagent | pCMV Lphn3-ΔCt | This paper | hLphn3 with a stop codon at residue 1117, causing deletion of the C-terminal tail (amino acids 1117–1447) | |
| Recombinant DNA reagent | pCMV Lphn3-T4L | This paper | hLphn3 with T4 lysozyme peptide inserted at Ile1039 in intracellular loop 3 | |
| Recombinant DNA reagent | pCAG Lphn2-T4L | This paper | rLphn2 with T4 lysozyme peptide inserted at Gly1045 in intracellular loop 3 | |
| Recombinant DNA reagent | Lentiviral Syn WT Lphn3 | PMID:30792275 | Lentiviral shuttle plasmid containing FLAG-tagged hLphn3 | |
| Recombinant DNA reagent | Lentiviral Syn Lphn3-ECD | This paper | Lentiviral shuttle plasmid containing Lphn3-ECD for rescue experiments in primary cultures | |
| Recombinant DNA reagent | Lentiviral Syn Lphn3-ΔCt | This paper | Lentiviral shuttle plasmid containing Lphn3-ΔCt for rescue experiments in primary cultures | |
| Recombinant DNA reagent | Lentiviral Syn Lphn3-T4L | This paper | Lentiviral shuttle plasmid containing Lphn3-T4L for rescue experiments in primary cultures | |
| Recombinant DNA reagent | Lentiviral Syn NLS-GFP-CRE p2a WT Lphn3 | PMID:30792275 | Lentiviral shuttle plasmid co-expressing NLS-GFP-Cre and WT hLphn3 for in vivo rescue experiments | |
| Recombinant DNA reagent | Lentiviral Syn NLS-GFP-CRE p2a Lphn3-T4L | This paper | Lentiviral shuttle plasmid co-expressing NLS-GFP-Cre and hLphn3-T4L for in vivo rescue experiments | |
| Recombinant DNA reagent | Lentiviral Syn NLS-GFP-CRE p2a WT Lphn2 | PMID:30792275 | Lentiviral shuttle plasmid co-expressing NLS-GFP-Cre and WT rLphn2 for in vivo rescue experiments | |
| Recombinant DNA reagent | Lentiviral Syn NLS-GFP-CRE p2a Lphn2-T4L | This paper | Lentiviral shuttle plasmid co-expressing NLS-GFP-Cre and rLphn2-T4L for in vivo rescue experiments | |
| Recombinant DNA reagent | Lentiviral EF1a phospho-PKA tdTomato | This paper | A PKA phosphopeptide substrate (MRKVSKGEGGRRVSN) was fused to the N-terminus of tdTomato separated by a linker (GGSGGGSGG) and was detected with the PKA phosphopeptide substrate antibody (sc-56941) | |
| Recombinant DNA reagent | pink Flamindo cAMP reporter | PMID:28779099; Addgene | RRID:addgene_102356 | |
| Recombinant DNA reagent | AAV CAG FLEX TVA-mCherry | PMID:26232228; Addgene | RRID:addgene_48332 | Rabies complementing AAV shuttle plasmid |
| Recombinant DNA reagent | AAV CAG FLEX rG | PMID:26232228; Addgene | RRID:addgene_48333 | Rabies complementing AAV shuttle plasmid |
| Antibody | Anti-HA mouse monoclonal | Covance | #MMS101R | 1:500 IHC; 1:1,000 ICC; 1:2,000 IB |
| Antibody | Anti-HA rabbit monoclonal | Cell Signaling Technologies | #3724 | 1:2,000 ICC; 1:1,000 IHC |
| Antibody | Anti-FLAG mouse monclonal | Sigma | #F3165 | 1:2,000 IB |
| Antibody | Anti-beta Actin mouse monoclonal | Sigma | #A1978 | 1:10,000 IB |
| Antibody | Anti-MAP2 chicken polyclonal | Encor | #CPCA MAP2 | 1:2,000 ICC |
| Antibody | Anti-PSD95 mouse monoclonal | Synaptic Systems | #124011 | 1:2,000 ICC |
| Antibody | Anti-vGLUT1 guinea pig polyclonal | Millipore | #AB5905 | 1:2,000 ICC |
| Antibody | Anti-Homer1 rabbit polyclonal | Synaptic Systems | #160003 | 1:2,000 ICC |
| Antibody | Anti-PKA phosphopeptide substrate mouse monoclonal | Santa Cruz Biotechnology | #sc-56941 | 1:1,000 ICC |
| Chemical compound, drug | Papain | Worthington | #LS003127 | |
| Chemical compound, drug | Matrigel | Corning | #356235 | |
| Chemical compound, drug | B-27 supplement | Gibco | #17504044 | |
| Chemical compound, drug | Cytosine arabinofuranoside | Sigma | #C6645 | |
| Chemical compound, drug | DAPI | Roche | #10236276001 | |
| Chemical compound, drug | QX-314 | Tocris | #1014 | |
| Chemical compound, drug | Picrotoxin | Tocris | #1128 | |
| Chemical compound, drug | Tetrodotoxin | Tocris | #1069 | |
| Chemical compound, drug | Freestyle MAX reagent | Life Technologies | #16447100 | |
| Software, algorithm | SnapGene | GSL Biotech | previously existing | |
| Software, algorithm | pClamp10 | Molecular Devices | previously existing | |
| Software, algorithm | Clampfit10 | Molecular Devices | previously existing | |
| Software, algorithm | NIS-Elements AR | Nikon | previously existing | |
| Software, algorithm | ImageJ | National Institutes of Health | previously existing | |
| Software, algorithm | Adobe Photoshop | Adobe | previously existing | |
| Software, algorithm | Adobe Illustrator | Adobe | previously existing | |
| Software, algorithm | Graphpad Prism 8.0 | Graphpad software | previously existing |