Skip to main content
. 2021 Mar 15;131(6):e139869. doi: 10.1172/JCI139869

Figure 10. Mutation of Lrp5 P847L in mice causes retinal vascularization defects.

Figure 10

(A) Images of P6 flat-mounted, IB4-stained retinas from Lrp5P847L/+ and Lrp5P847L/P847L mice. Compared with those of Lrp5P847L/+ mice, retinas from Lrp5P847L/P847L mice showed incomplete vascularization. Scale bars: 500 μm. (B) Quantification of the vascular progression in retinas from Lrp5P847L/+ and Lrp5P847L/P847L mice on P6. Error bars indicate the SD. ****P < 0.0001, by Student’s t test (n = 8). (C) DAPI staining of hyaloid vessels in the eyes of Lrp5P847L/+ and Lrp5P847L/P847L mice, showing delayed hyaloid vessel regression in the eyes of Lrp5P847L/P847L mice. Scale bars: 250 μm. (D) Frozen retinal sections from P9 Lrp5P847L/+ and Lrp5P847L/P847L mice were costained with IB4 (red) and DAPI (blue). Scale bars: 25 μm. Experiments were performed independently at least 3 times.