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. Author manuscript; available in PMC: 2021 Mar 12.
Published in final edited form as: Science. 2020 Jul 10;369(6500):161–167. doi: 10.1126/science.aax2517

Fig. 4. Pharmacological impact of Tx24 for the dissociation of the orthosteric antagonist.

Fig. 4.

(A) Comparison of the [3H]NMS dissociation kinetics from M2AChR (blue), M1AChR (red), M3AChR (magenta), M4AChR (green), or M5AChR (orange) in the absence (filled symbols, solid line), or presence (empty symbol, solid line) of 2 μM Tx24. Shown are the combined results from four assays performed in duplicate. (B) Saturation binding of [3H]NMS to M2AChR in the absence (filled symbol, solid line) or presence (empty symbol, dotted line) of 0.5 μM Tx24 after 24-hour incubation. Shown are the combined results from four assays performed in duplicate. (C) ACh-stimulated [35S]GTP-γ-S binding at M2AChR expressed in CHO cells in the absence (filled symbol, solid line) or presence (empty symbol, dotted line) of 1 μM Tx24. Symbols and error bars represent means and SEM of the combined results from three or four assays performed in duplicate.