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. 2020 Dec 9;184(1):133–148.e20. doi: 10.1016/j.cell.2020.12.005

Figure 2.

Figure 2

TMEM41B Is a Pan-flavivirus Host Factor

(A–D) (A) WT and TMEM41B KO HAP1 cells infected with encephalitic or hemorrhagic fever tick-borne flaviviruses. (C) WT and TMEM41B KO Huh-7.5 clones infected with HCV. (D) WT and TMEM41B KO MDBK clones infected with BVDV. For (C) and (D) dots represent the average of n = 3 replicates from individual single cell clones. Virus strain is indicated in the figure. Error bars in (A) and (B) show SD for n = 6 replicates. Error bars in (C) and (D) show SD for n = 3 replicates (WT) and individual KO clones.

(E) Table summarizing results shown in Figure 2 (A–D), Figure S3 (A, B, D, F), and Figure S4 (A–D) from infection experiments with multiple viruses in various mammalian and mosquito cell lines. TBFV, tick-born flaviviruses of (A) and (B); +++, infection comparable to WT cells; +/−, reduced but detectable infection; −, negligible infection.

(F–I) WT, TMEM41B KO, and reconstituted HAP1 cells infected with (−) sense RNA viruses (F); VSV-G-pseudotyped HIV-1 lentivirus (LV-GFP) (G); (+) sense RNA viruses (H); or DNA viruses (I). Cells were analyzed by flow cytometry and plotted as a percentage of viral antigen-positive cells or GFP/RFP-positive cells for reporter viruses expressing a fluorescent protein. Error bars depict SD for n = 3 replicates or the indicated number of clones.