Figure 5.

TMEM41B Co-localizes with Flavivirus NS4A and NS4B Proteins

(A) TMEM41B KO HAP1 cells expressing RFP-TMEM41B visualized in uninfected cells (mock) and YFV- and ZIKV-infected cells 24 h post infection. Anti-NS4A (ZIKV) and anti-NS4B (YFV) antibodies detect viral antigens. Yellow-orange color in the merged column shows the co-localization of TMEM41B with viral antigens. DAPI stains cell nuclei.

(B) Western blot shows that an anti-RFP antibody which recognizes RFP-TMEM41B (but not an IgG antibody control) co-immunoprecipitates ZIKV NS4A in HAP1 cells.

(C) Same as (B) but with YFV infection and visualized with an antibody that detects YFV NS4B.

WCL, whole cell lysate. Of note, the heavy chain of the capture antibodies is detected by the secondary antibody protein A-HRP as indicated ~52 kDa. See also Figure S5.