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. 2020 Dec 9;184(1):133–148.e20. doi: 10.1016/j.cell.2020.12.005

Figure S4.

Figure S4

Flavivirus Infections in Mosquito TMEM41B KO Cells, Related to Figure 2

(A–C) WT and TMEM41B KO and KO reconstituted Aag2 and C6/36 mosquito cell clones generated with independent sgRNAs infected with: ZIKV, MOI = 0.05 PFU/cell for 72 h; DENV, MOI = 0.2 PFU/cell for 96 h; WNV-GFP, MOI = 10 PFU/cell for 72 h.

(D and E) Same as A-C but without TMEM41B reconstitution. YFV 17D, MOI = 10 PFU/cell for 72 h; YFV Asibi, MOI = 0.5 PFU/cell for 72 h; CHIKV, MOI = 0.1 PFU/cell for 72 h (Aag2) and 0.05 PFU/cell for 48 h (C6/36). CHIKV was included as a non-flavivirus control.

(F) Western blot to detect TMEM41B and VMP1 in lysates from indicated cell lines. β-actin is included as a loading control. Cells were analyzed by flow cytometry and plotted as a percentage of viral antigen positive cells or GFP/RFP positive cells for reporter viruses expressing a fluorescent protein. Panels A-E, error bars depict SD for n=3 replicates or the indicated number of clones.