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. 2020 Dec 10;147(4):1031–1040. doi: 10.1007/s00432-020-03486-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Representative images of immunohistochemistry (IHC) and fluorescence in-situ hybridization (FISH) analysis for the evaluation of GATA6 gene copy numbers using the GATA6-20-GR (green) and the Zytolight centromere 18 (CEN18) (red) Probe illustrating (upper row) GATA6-positive versus (lower row) GATA6-negative esophageal adenocarcinoma. GATA6 amplification was defined as gene copy cluster > 50% of the tumor cells, respectively, gene copy number > 6 per cell