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. 2020 Nov 19;109(2):524–539. doi: 10.1007/s10266-020-00569-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Effect of adhesive extracts on cell cycle (a) and related gene expression (b). Human gingival fibroblasts were treated with undiluted extracts for 24 h. Cells were collected and stained with PI and analyzed by flow cytometry for percentage of cell in different phases of cell cycle (a). p16 and p21 gene expression were evaluated by RT-PCR (b). The values represent the mean ± SD of three independent experiments performed in quintuplicate for each dental material. Differences vs. control: *p < 0.05; **p < 0.001