Table 2.

Primer sequences used for RT-PCR analysis

mRNA	Sequences (5′–3′)	Product (bp)
GAPDH	Fw: TGGTATCGTGGAAGGACTCATGAC Rv: ATGCCAGTGAGCTTCCCGTTCAGC	188
Fibronectin	Fw: TTTTGAGAGCTGATGACAGACA Rv: GCTCTTAATGGCAGAGAGGA	150
Collagen type I	Fw: GTGAGACAGGCGAACAGG Rv: GACCAGCAGGCACAGAGG	129
MMP1	Fw: TACACGCCAGATTTGCCAAG Rv: ATGAGCAAGATTTCCTCCAG	189
MMP2	Fw: CTGGAGAACTAGAGAAGGAC Rv: GAGGAGTACAGTCAGCATCT	147
MMP12	Fw: TGCTGATGACATACGTGGCA Rv: AGGATTTGGCAAGCGTTGG	69
Elastin	Fw: CTGGAATTGGAGGCATCG Rv: ACCTGGGACAACTGGAAT	200
VEGF	Fw: TGCTGTCTTGGGTGCATTGG Rv: GGTGCAGCCTGGGACCACT	72
IL-1β	Fw: GGACCTGGACCTCTGCCCTCTGG Rv: GCCTGCCTGAAGCCCTTGCTGTAG	80
IL-6	Fw: CAGAACAGATTTGAGAGTAGTGA Rv: CGCAGAATGAGATGAGTTGT	200
IL-8	Fw: GACATACTCCAAACCTTTCCA Rv: AACTTCTCCACAACCCTCT	162
Bcl-2	Fw: AGATGTCCAGCCAGCTGCACCTGAC Rv: AGATAGGCACCCAGGGTGATGCAAGCT	366
p53	Fw: GGACCTGATTTCCTTACTG Rv: TGAATCTGAGGCATAACTG	248
p21	Fw: TGGAGACTCTCAGGGTCGAA Rv: GACTGCAGGCTTCCTGTGG	118
p16	Fw: CCCAACGCACCGAATAGTTA Rv: CACCAGCGTGTCCAGGAA	173