Fig. 3.

Low doses of RT increase IL-10 production by immune cells in H1N1 pneumonia model. Mice were treated with PR8 influenza virus (H1N1) or PBS by intranasal instillation. After 2 days, mice were irradiated at the whole thorax at 0.5 Gy or 1 Gy or treated with dex. (A) At 3 days after PR8 infection, the percentages of IL-10+ NAMs are presented for each treatment group (left), and representative histograms of the mean fluorescence of IL-10 and t-SNE maps of IL10 expression in NAMs are shown (middle and right). (B) The percentages of IL-10+ AMs are presented for each treatment group (left), and representative histograms of the mean fluorescence of IL-10 and t-SNE maps of IL10 expression in AMs are shown (middle and right). (C) The percentages of IL-10+ neutrophils are presented for each treatment group (left), and representative histograms of the mean fluorescence of IL-10 and t-SNE maps of IL-10 expression in neutrophils are shown (middle and right). Data are from 2 independent experiments (n = 6). For all data, bars indicate means and error bars indicate ±SEMs. *P < .05, **P < .01 (1-way ANOVA). Abbreviations: AM = alveolar macrophage; ANOVA = analysis of variance; dex = dexamethasone; Gy = Gray; IL = interleukin; NAM = nerve- and airway-associated macrophage; PBS = phosphate-buffered saline; RT = radiation therapy; SEM = standard error of the mean; t-SNE = t-distributed stochastic neighbor embedding.