Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 12;11:5871. doi: 10.1038/s41598-021-85415-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

High resolution gene expression analysis detected dynamic spatiotemporal localization of rspo2 and rspo3 transcripts in zebrafish cranial mesenchyme. (A) Whole-mount RNA in situ hybridization detected rspo2 and rspo3 transcripts in the midbrain (mb), forebrain (f), otic vesicle (o), and Meckel’s cartilage (arrowhead) at 24 and 48 hpf in lateral and dorsal views. Transcript of rspo3 was additionally detected at 24 and 48 hpf in the forebrain (f) and at 48 hpf in the ethmoid plate (open arrowhead). Scale bar: 100 um. (B) Maximum projections of z-stacks of coronal sections of zebrafish embryos, section in plane with eyes. Labeled schematic at left. At 48 hpf, rspo2 and rspo3 transcripts were highly co-localized in the mesenchyme surrounding the condensing trabeculae cartilage mesenchyme, marked by runx2a expression. rspo2 is also detected within the condensing mesenchyme at this timepoint. At 5 dpf rspo2 and rspo3 continue to be co-expressed in mesenchyme and perichondrium surrounding cartilage elements, specifically the ethmoid plate and Meckel’s cartilage. Rspo3 expression is particularly high in runx2a expressing osteogenic precursor cells associated with Meckel’s cartilage, as well as where the palatoquadrate meets the ethmoid plate (white arrowhead). Abbreviations: e: eye, o: olfactory organ, s: stomodeum, t: trabeculae. Scale bar: 100 μm.