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. 2021 Mar 12;11:5871. doi: 10.1038/s41598-021-85415-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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rspo2 and rspo3 are differentially expressed in zebrafish pharyngeal teeth. Representative images of maximum projections of z-stacks of sagittal sections of 180 dpf zebrafish. rspo2 (red) and rspo3 (white) transcripts were detected by RNAscope in situ hybridization. rspo3 is diffusely expressed throughout the supporting mesenchyme and highly expressed in enamel epithelium (open arrow) and some tooth pulp (closed arrow). Often within the tooth pulp, rspo3 expression is restricted to the outermost odontoblasts (*). Meanwhile, rspo2 expression is highest in enamel epithelium (open arrow) and very low within the tooth pulp. Scale bar: 100 μm.