Figure 5.

DUSP3 deletion associated with HFD strongly promote DEN-induced hepatocarcinogenesis. DEN (25 mg/kg) was i.p injected to 14-day-old DUSP3-KO and WT mice. Four weeks later, diet of the mice was switched to CD or HFD and weight was monitored every two weeks for 31 weeks (a). (b) Livers of WT and mutant mice kept on CD or HFD 24 and 32 weeks after the administration of DEN. (c–e) Tumor multiplicity (c), size (d), and SAF score evaluation (e) in livers of DEN-injected WT and DUSP3-KO mice kept on CD or HFD as above. Each dot represents the average number of tumors or size of the tumors for one mouse. Results are means ± SD. Each dot represents one mouse. Mean ± SD values for all mice are shown. *P < 0.05; **P < 0.01. n = 10 mice in each group.