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. 2021 Mar 12;12:1657. doi: 10.1038/s41467-021-21802-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a DII ratiometric signal in the indicated YUC-TAA1 R2D2 lines in the aux1 background. Presented are merged confocal images of mDII (red) and DII (yellow). Scale bar = 100 μm. b Quantification of relative DII/mDII following 5 μM estradiol and mock treatments of indicated aux1-mutant lines. The red boxes indicate regions used to quantify the DII/mDII ratio. Shown are means (±SE) of two independent roots, each with two regions of interest (n = 4). c Quantification of root nuclei instantaneous speed following 5 μM estradiol treatment of the indicated aux1-mutant lines. Data points are concatenated means (±SD) of ~1000 nuclei from two independent root movies, n ≥ 125 nuclei. d DII ratiometric signal in the indicated YUC-TAA1 R2D2 lines in the pin2 background. Presented are merged confocal images of mDII (red) and DII (yellow). Scale bar = 100 μm. e Quantification of relative DII/mDII following 5 μM estradiol and mock treatments of indicated pin2-mutant lines. The blue box indicates the region used to quantify the DII/mDII ratio. Shown are means (±SE) of two independent roots, n ≥ 300 nuclei. f Quantification of root nuclei instantaneous speed following 5 μM estradiol treatment of indicated pin2-mutant lines. Data points are concatenated means (±SD) of ~1000 nuclei from two independent root movies. g–h Quantification of the root nuclei instantaneous angles in the indicated lines following treatment with 5 μM estradiol or untreated mock control. Data points are concatenated means (±SD) of ~1000 nuclei from two independent root movies. i–j Single-cell scatter analysis of velocity at the X and Z-dimensions. Time is indicated by the rainbow scale. T₀ is 10 min following mock or estradiol treatment. Ctr indicates controls, heterozygous lines for promoter-specific:XVE:YUC1:TAA1; R2D2 and the aux1 (i) or the pin2 (j) mutation. aux1 or pin2 are also heterozygous for promoter-specific:XVE:YUC1:TAA1; R2D2 but homozygous for aux1 or pin2. Statistical analysis is presented in Supplementary Fig. 17. For all experiments, the n number for each line and time point is indicated at Source data file.