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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Mucosal Immunol. 2020 Oct 1;14(2):479–490. doi: 10.1038/s41385-020-00347-6

Figure 5. Dietary UDCA supplementation in DSS-treated WT and Mdr2−/− mice.

Figure 5.

Ten-week-old mice were fed 0.5% UDCA supplemented chow for 2 weeks prior to and throughout the DSS treatment and recovery. For DSS treatment, mice were given 1.5% DSS in drinking water for 5 days and then allowed to recover. Tissues and sera were collected upon euthanasia on day 9 after DSS. (A) Scheme of treatment. (B) Body weight curves over the DSS treatment and recovery. (C) Disease activity measured by combining weight loss, stool consistency, and bleeding. (D and E) Representative colon histology (H&E; 100X) and histological scores. (F) Colon length at time of euthanasia divided by the starting body weight. (G) Colon tissue was homogenized, and cytokine protein levels were measured by Meso Scale Discovery analysis. (H) RT-qPCR analysis of adhesion molecules after UDCA and DSS treatment. (I) Colonic MAdCAM-1 protein levels were measured using an ELISA kit. n ≥ 8 mice per group. Data are expressed as means ± SEM. *P<0.05, ***P<0.0001, two-way ANOVA.