Table 1. Summary of protein localization within endothelial signaling domains.
This table summarizes protein localization to one or multiple endothelial signaling compartments that have been described in the text. Although this review focuses on localization within the resistance artery endothelium, there are a limited number of studies that use this model system and for this reason, localization data from other model systems are included (1) to provide a more complete summary of the body of literature on endothelial protein localization, and (2) to highlight the need for more studies in small arteries. Since endothelial cells have unique physiological roles based on vessel type, it is crucial that localization studies be performed in the arteriole endothelium to determine if there are differences compared to large arteries or even veins.
| Full Name | Source: Tissue | Source: Cell Culture | Luminal | Abluminal | Interendothelial | |
|---|---|---|---|---|---|---|
| A kinase anchoring protein 150 | AKAP 150 | Mouse mesenteric artery(128) | (128) | |||
| A kinase anchoring protein 12 | AKAP 12 | HDMEC(202) | (202) | |||
| A kinase anchoring protein 9 | AKAP 9 | HUVEC(276) | (276) | |||
| Alkaline phosphatase | ALP | Rat BCEC(334) | (334) | (334) | ||
| Angiotensin converting enzyme | ACE | Canine vasculature(60) Rabbit vasculature(61) Aorta & pulmonary arteries (bovine, pig & rabbit)(62) Human carotid artery(63) |
(60–63) | |||
| Bradykinin B2 Receptor | Rat aorta(50) | (50) | ||||
| Calnexin | Caln | Third order mesenteric artery(186) | (186) | |||
| Calreticulin | Calr | Third order mesenteric artery(130) | HCAEC(56) | (56, 130) | ||
| Caveolin 1 | Cav1 | Mouse carotid(335) Mouse mesenteric artery(278) |
HCAEC(56) HMVEC(286) BCAEC(287) |
(286, 287, 335) | (278) | |
| Claudin-5 | Cldn5 | Mouse mesenteric artery | Endothelial cells derived from murine ESC(242) | (242) | ||
| Connexin37 | Cx37 | Rat mesenteric artery(159) Mouse mesenteric artery(278) Mouse cremaster arterioles(167) Hamster cheek pouch arteriole(262, 263) |
(159, 167) | (159, 262, 278) | ||
| Connexin40 | Cx40 | Rat mesenteric artery(159, 169) Mouse mesenteric artery(278) Mouse cremaster arteriole(167) Hamster cheek pouch arteriole(262, 263) |
(167, 169) | (159, 262, 263, 278) | ||
| Connexin43 | Cx43 | Rat mesenteric artery(159) Mouse mesenteric artery(278) Mouse cremaster arteriole(167) Hamster cheek pouch arteriole(262, 263) |
(167) | (159, 262, 263, 278) | ||
| E-Selectin | CD62 | Primary HBMEC(4) | (4) | |||
| Endothelial nitric oxide synthase | eNOS | Mouse carotid(336) Mouse thoracodorsal artery(337) |
Primary HCAEC(56) | (56, 336) | (56),(337) | |
| Endothelin B Receptor | ETB | Primary HAoEC(338) | (338) | (338) | ||
| Filamentous actin | F-actin | Mouse cremaster arterioles(167) | HUVEC(274) VCCC(189, 190) |
(167, 189, 190) | (274) | |
| Hemoglobin alpha | Alpha globin | Mouse thoracodorsal artery(124) | HCAEC(125) | (124) | (124, 125) | |
| Heparan sulfate proteoglycan | HSPG | Bovine aorta(217) | Porcine AoEC(339) HAoEC(340) Primary BAoEC(341) HUVEC-pericyte tube co-assembly model(138) |
(340, 341) | (138, 217, 339) | |
| Inositol trisphosphate receptor 1 | IP3R1 | Mouse mesenteric artery(186) | VCCC(132, 133) | (132, 133, 186) | ||
| Integrin α1β1 | HUVEC(211) | (211) | ||||
| Integrin α2β1 | HUVEC(206, 278) | (278) | (206, 278) | |||
| Integrin α5β1 | HUVEC(206, 216, 278) | (216) | (206, 278) | |||
| Integrin αvβ3 | HUVEC (207, 211, 216) | (207, 211, 216) | ||||
| Intermediate conductance | IKCa | Rat cremaster arteriole(294) | (159, 294) | |||
| calcium-activated potassium channel | Rat mesenteric artery(159) | |||||
| Muscarinic M3 Receptor | mAChR | Human cerebral artery(38) | (38) | |||
| N-Cadherin | NCAD | Mouse cremaster arterioles(167) | (167) | |||
| Occludin | Ocln | Rat diaphragm arteriole(237) Rat aorta, mesenteric artery, renal vein, vena cava(238) |
(237, 238) | |||
| Plasminogen activator inhibitor 1 | PAI-1 | VCCC(189–191) | (189–191) | |||
| Platelet endothelial adhesion molecule 1 | PECAM-1 or CD31 | Mouse aorta(272) | HUVEC(271, 273, 274) | (271–274) | ||
| Plakoglobin | γ-catenin | Primary porcine AoEC(305) HDMEC(245) |
(245, 305) | |||
| Purinergic Receptor P2Y1 | P2Y1 | Rat sinus endothelium(114) | (114) | |||
| Purinergic Receptor P2Y6 | P2Y6 | Rat sinus endothelium(114) | (114) | |||
| Sarco/endoplasmic reticulum Ca2+-ATPase | SERCA | Mouse thoracodorsal artery(185) | (185) | |||
| S-endo-1-associated antigen | CD146 | HUVEC(274) | (274) | |||
| Single chain glycoprotein 1 | CD99 | HUVEC(273) | (273) | |||
| Small conductance calcium-activated potassium channel 3 | SK3 | Rat cremaster arteriole(297) Rat mesenteric artery(159) |
(297) | (297) | (159) | |
| Sodium-coupled neutral amino acid transporter 3 | Snat3 | Mouse abdominal aorta(342) | (342) | (342) | ||
| Sphingosine-1- phosphate receptor | S1P | Rat brain capillary endothelium(343) | (343) | (343) | ||
| Thrombomodulin | TM | HUVEC(118, 344) | (118, 344) | (344) | ||
| Transient receptor potential channel subfamily C member 1 | TRPC1 | Human mesenteric artery(345) | HPAEC(346) | (345) | (346) | |
| Transient receptor potential channel subfamily C member 4 | TRPC4 | HMVEC(347) | (347) | (347) | ||
| Transient receptor potential channel subfamily V member 4 | TRPV4 | Mouse 3rd order mesenteric artery(131) Mouse carotid artery(348) Rat cremaster arteriole(294) |
(348) | (131, 294) | ||
| Vascular endothelial cadherin | VE-Cadherin | Mouse carotid artery(249) Mouse diaphragm capillaries(249) Mouse bladder capillaries(249) |
HUVEC(249, 273, 274) | (249, 273, 274) |
Abbreviations: Bovine aortic endothelial cells (BAoEC), Bovine coronary artery endothelial cells (BCAEC), Brain capillary endothelial cells (BCEC), Embryonic stem cells (ESC), Human aortic endothelial cells (HAoEC), Human brain microvascular endothelial cells (HBMEC), Human coronary artery endothelial cells (HCAEC), Human dermal microvascular endothelial cells (HDMEC), Human microvascular endothelial cells (HMVEC), Human pulmonary artery endothelial cells (HPAEC), Human umbilical vein endothelial cells (HUVEC), Vascular cell coculture (VCCC)