Table 6.
Identification of phosphorylation, ubiquitination, and acetylation in growth hormone of growth hormone-secreting PA and controls
| Quantitative proteomics identified modifications in growth hormone | Modifications in growth hormone proteoforms | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene names | Swiss-Prot | Modificed peptide | Modified peptide position | Modified residue | Modified residue position | Calculated unmodified peptide (m/z) | Observed unmodified peptide (m/z; PMF data) | Calculated modified peptide (m/z) | Observed modified peptide (m/z; PMF data) | Proteoforms (2DE spot No.) | Detected PTM | Methods |
| GH1 | P01241 | SVFANSLVYGAS*DSNVYDLLK | 121–141 | S132 | P132 | 2262.13 | 2342.13 | 2342.20 | T12 | + | PMF + MS/MS | |
| 2262.30 | 2342.30 | T36 | + | PMF + MS/MS | ||||||||
| 2342.20 | T54 | + | PMF + MS/MS | |||||||||
| 2262.60 | 2342.60 | C11 | + | PMF + MS/MS | ||||||||
| 2262.60 | 2342.70 | C32 | + | PMF + MS/MS | ||||||||
| 2262.70 | 2342.80 | C33 | + | PMF + MS/MS | ||||||||
| 2262.20 | 2342.20 | C56 | + | PMF + MS/MS | ||||||||
| YSFLQNPQTS*LCFSESIPTPSNR | 68–90 | S77 | P77 | 2616.24 | 2696.24 | 2696.05 | T9 | + | PMF | |||
| 2696.18 | T10 | + | PMF | |||||||||
| 2696.27 | T13 | + | PMF | |||||||||
| 2696.14 | T16 | + | PMF | |||||||||
| 2696.17 | T17 | + | PMF | |||||||||
| 2696.44 | T33 | + | PMF | |||||||||
| 2696.11 | T38 | + | PMF | |||||||||
| 2696.14 | T43 | + | PMF | |||||||||
| 2696.27 | T44 | + | PMF | |||||||||
| FDTNS*HNDDALLK | 172–184 | S176; N178 | P176; D 178 | 1489.69 | 1489.65 | 1569.69 | 1570.81 | T6 | + | PMF | ||
| 1489.70 | 1569.85 | T39 | + | PMF | ||||||||
| 1489.71 | 1570.92 | T78 | + | PMF | ||||||||
| QTYSKFDTNS*HNDDALLK | 167–184 | 2096.99 | 2096.95 | 2176.99 | - | |||||||
| FDTNS*HNDDALLKNYGLLYCFR | 172–193 | 2619.23 | 2619.22 | 2699.23 | - | |||||||
| TGQIFKQTYSKFDTNS*HNDDALLK | 161–184 | 2771.36 | 2771.26 | 2851.36 | - | |||||||
| FDT*NSHNDDALLK | 172–184 | T174; N178 | P174; D 178 | 1489.69 | 1489.65 | 1569.69 | 1570.81 | T6 | + | PMF | ||
| 1489.70 | 1569.85 | T39 | + | PMF | ||||||||
| 1489.71 | 1570.92 | T78 | + | PMF | ||||||||
| QTYSKFDT*NSHNDDALLK | 167–184 | 2096.99 | 2096.99 | 2176.99 | - | |||||||
| FDT*NSHNDDALLKNYGLLYCFR | 172–193 | 2619.23 | 2619.22 | 2699.23 | - | |||||||
| TGQIFKQTYSKFDT*NSHNDDALLK | 161–184 | 2771.36 | 2771.36 | 2851.36 | - | |||||||
| TGQIFKQTYSK* | 161–171 | K171 | Acet-K171 | 1300.69 | 1300.18 | 1342.69 | - | |||||
| LEDGSPRTGQIFKQTYSK* | 154–171 | 2055.05 | 2055.03 | 2097.05 | - | |||||||
| TGQIFKQTYSK*FDTNSHNDDALLK | 161–184 | 2771.36 | 2771.36 | 2813.36 | - | |||||||
| QTYSK* | 167–171 | 626.31 | 626.51 | 668.31 | - | |||||||
| QTYSK*FDTNSHNDDALLK | 167–184 | 2096.99 | 2096.68 | 2138.99 | - | |||||||
| QTYSK*FDTNSHNDDALLKNY GLLYCFR | 167–193 | 3226.53 | 3226.56 | 3268.53 | 3267.79 | T30 | + | PMF | ||||
| EETQQK*SNLELLR | 91–103 | K96 | Ub-K96 | 1587.83 | 1587.81 | 1701.83 | 1701.93 | T78 | + | PMF | ||
| EETQQK* | 91–96 | 762.36 | 762.16 | 876.36 | - | |||||||
| EETQQK*SNLELLRISLLLIQSWLEPVQFLR | 91–120 | 3624.02 | 3624.13 | 3738.02 | - | |||||||
| YSFLQNPQTSLCFSESIPTPSNREETQQK* | 68–96 | 3359.59 | 3359.28 | 3473.59 | - | |||||||
| FDTNSHN*DDALLK | 172–184 | N178 | DEAM 178 | 1489.69 | 1489.67 | 1490.69 | 1490.65 | T1 | + | PMF | ||
| 1489.65 | 1490.69 | 1490.66 | T5 | + | PMF | |||||||
| 1489.65 | 1490.69 | 1490.65 | T6 | + | PMF | |||||||
| 1489.72 | 1490.69 | 1490.72 | T8 | + | PMF | |||||||
| 1489.69 | 1490.69 | 1490.67 | T9 | + | PMF | |||||||
| 1489.75 | 1490.69 | 1490.75 | T13 | + | PMF | |||||||
| 1489.71 | 1490.69 | 1490.70 | T14 | + | PMF | |||||||
| 1489.71 | 1490.69 | 1490.70 | T16 | + | PMF | |||||||
| 1489.71 | 1490.69 | 1490.72 | T17 | + | PMF | |||||||
| 1489.77 | 1490.69 | 1490.74 | T18 | + | PMF | |||||||
| 1489.72 | 1490.69 | 1490.72 | T28 | + | PMF | |||||||
| 1489.77 | 1490.69 | 1490.77 | T30 | + | PMF | |||||||
| 1489.79 | 1490.69 | 1490.80 | T34 | + | PMF | |||||||
| 1489.72 | 1490.69 | 1490.73 | T36 | + | PMF | |||||||
| 1489.67 | 1490.69 | 1490.68 | T38 | + | PMF | |||||||
| 1489.70 | 1490.69 | 1490.70 | T39 | + | PMF | |||||||
| 1489.75 | 1490.69 | 1490.71 | T43 | + | PMF | |||||||
| 1489.86 | 1490.69 | 1490.81 | T44 | + | PMF | |||||||
| 1489.75 | 1490.69 | 1490.74 | T53 | + | PMF | |||||||
| 1489.69 | 1490.69 | 1490.67 | T54 | + | PMF | |||||||
| 1489.69 | 1490.69 | 1490.78 | T57 | + | PMF | |||||||
| 1489.68 | 1490.69 | 1490.68 | T58 | + | PMF | |||||||
| 1489.74 | 1490.69 | 1490.79 | T59 | + | PMF | |||||||
| 1489.79 | 1490.69 | 1490.79 | T63 | + | PMF | |||||||
| 1489.62 | 1490.69 | 1490.60 | T67 | + | PMF | |||||||
| 1489.70 | 1490.69 | 1490.70 | C14 | + | PMF | |||||||
| 1489.68 | 1490.69 | 1490.68 | C56 | + | PMF | |||||||
+ : this PTM was detected. -: this PTM was not detected. ± : this PTM cannot be determined