KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Experimental Models: Cell lines | ||
| Human Caco-2 | ATCC | Cat. No. HTB-37 |
| Human HepG2 | ATCC | Cat. No. HB-8085 |
| Mouse Hepa 1-6 | ATCC | Cat. No. CRL-1830 |
| Human NCI-H716 | ATCC | Cat. No. CCL-251 |
| 16S sequencing data for mouse sham and SG cecal, and human pre- and post-SG fecal samples | NCBI-SRA | BioProject ID PRJNA635168 |
| Experimental Models: Animals | ||
| Diet-induced obese mice | Jackson Labs | https://www.jax.org/ |
| GF mice (tissue analysis and cecal microbial transplant) | This study | https://metagenomics.partners.org/ |
| GF C57BL/6NCrl mice (LCA feeding) | This study | Huh Lab, HMS |
| Vdr-KO and WT animals | This study | Demay Lab, MGH |
| Sham and SG animals | This study | Sheu Lab, BWH |
| Experimental Models: Human samples | ||
| Human patient pre-SG and post-SG feces samples | This study | Sheu Lab, BWH |
| Critical Commercial Assays | ||
| Cell Titer-Glo Luminescent Cell Viability Assay | Promega | Cat. No. G7570 |
| GLP-1 ELISA kit | Sigma | Cat. No. RAB0201 |
| Deposited Data | ||
| 16S Sequencing data - SG and sham mouse cecal, and pre-SG and post-SG human fecal microbiome analysis | NCBI-SRA | BioProject ID PRJNA635168 |
| Recombinant DNA | ||
| Human VDR siRNA | Santa Cruz Biotechnology | Cat. No. sc-106692 |
| Human PXR siRNA | Santa Cruz Biotechnology | Cat. No. sc-44057 |
| Human FXR siRNA | Santa Cruz Biotechnology | Cat. No. sc-38848 |
| Human CAR siRNA | Santa Cruz Biotechnology | Cat. No. sc-29908 |
| Human RORα siRNA | Santa Cruz Biotechnology | Cat. No. sc-38862 |
| Human RORγ siRNA | Santa Cruz Biotechnology | Cat. No. sc-38880 |
| Oligonucleotide primer sequences | This study; (Wells et al., 2003) | Table S1; doi: 10.1016/s0009-8981(03)00115-3 |
| Software and Algorithms | ||
| Nephele - 16S sequencing analysis | (Weber et al., 2018) | https://nephele.niaid.nih.gov/about/ |
| GraphPad Prism 7 | Commercial Software | https://www.graphpad.com/scientific-software/prism/ |
| Other | ||
| Animal diet - HFD, DIO mice | Research Diets Inc | Cat. No. D12492 |
| Animal diet - HFD, GF mice | Research Diets Inc | Cat. No. D12492ii |
| Animal diet - Standard Chow, GF mice | PicoLab Diet | Cat. No. 5053 |
| Animal diet - Rescue Chow, WT and Vdr-KO mice | Teklad | Cat. No. TD96348 |