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. 2021 Mar 12;4:332. doi: 10.1038/s42003-021-01857-0

Fig. 2. The transcription factor Creb5 is differentially expressed in superficial versus deep zone articular chondrocytes and is specifically expressed in Prg4+ cells in synovial joints.

Fig. 2

a RT-qPCR analysis of Prg4 and Creb5 expression in either SZCs or DZCs cultured in either the absence (white) or presence (gray) of TGF-β2 (20 ng/ml) for 3 days. Gene expression was assayed by RT-qPCR and normalized to Gapdh. Error bar indicates standard error of the mean (n = 2 technical repeats). Similar results have been obtained in three independent biological repeats. b Western analysis of proteins in SZCs and DZCs. Similar results have been obtained in two independent biological repeats. c Immunofluorescent staining for CREB5, lubricin, and DAPI (to visualize nuclei) in adult human femoral head articular cartilage. Scale bar equals 50 microns. d Schematic of Prg4 expression in a developing long bone cartilage element. e Expression of Collagen 2a1 (Col2), Prg4, Creb5, and Matrilin1 (Matn1) in the elbows of P0 mice, as detected by fluorescent in situ hybridization. f Immunofluorescent staining for Creb5 protein and DAPI (to visualize nuclei) in the knee joint of a P0 mouse. In (e, f), the Creb5-expressing articular perichondrium is designated by a yellow arrow, the metaphyseal perichondrium is designated by a white arrow; and the scale bars equal 100 microns.