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. 2021 Mar 8;13:2235–2246. doi: 10.2147/CMAR.S282269

Figure 1.

Figure 1

Establishment of SLC39A4 stably knocked-down cell lines in GBC-SD cells and NOZ cells. (A and B) Knockdown efficiency of three shSLC39A4s was evaluated in GBC-SD cells by real-time RT-qPCR (A) and Western blotting (B) and shSLC39A4-2 was selected to carry out subsequent experiments. (C and D) Confirmation of SLC39A4 expression in NOZ shSLC39A4-2 (NOZ/shSLC39A4-2) and control (NOZ/shCtrl) cells by real-time RT-qPCR (C) and Western blotting (D). The results of real-time RT-qPCR are the mean ± SD (unpaired t-test). *p < 0.05, **p < 0.01, ***p < 0.001, compared with NOZ/shCtrl or GBC-SD/shCtrl.