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. 2021 Mar 8;13:2235–2246. doi: 10.2147/CMAR.S282269

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© 2021 Li et al.

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Overexpression of SLC39A4 promoted the growth and migration of cells. (A) Real-time RT-qPCR was done to confirm expression of exogenous SCL39A4 in GBC-SD cells and NOZ cells. mRNA expression was normalized to that of GAPDH. (B) MTT assays were undertaken to evaluate cell proliferation. (C–F) Cycle distributions were analyzed in GBC-SD cells and NOZ cells overexpressing SLC39A4 by FACS (C and E) and data are presented in (D and F) normalized to the total cell number. (G–J) Transwell-migration assays were conducted to observe the transmigration capability of NOZ/SLC39A4 cells and GBC-SD/SLC39A4 cells. (G and I) Representative images (magnification, ×200) and (H and J) quantitation are shown. Data are the mean ± SD (unpaired t-test). *p < 0.05, **p < 0.01, ***p < 0.001, compared with control cells.