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. 2021 Mar 12;12:1654. doi: 10.1038/s41467-021-21921-x

Fig. 2. A-to-I editing activity of ADAR1p110, not ADAR1p150 or ADAR2, is required for suppression of R-loops.

Fig. 2

a–e Dot blot analysis for RNA:DNA hybrids was conducted using control oligos (a) or genomic DNA (b–e). a The S9.6 antibody recognized specifically RNA:DNA but not DNA:DNA or RNA:RNA oligo duplex controls. b, c Increased RNA:DNA hybrids were detected only in ADAR1-depleted but not in ADAR2-depleted HeLa cells. b The S9.6 antibody signals were abolished by E. coli-RNase H treatment, confirming specific detection of RNA:DNA hybrids. d Comparison of RNA:DNA hybrid levels between depletion of ADAR1 versus depletion of known R-loop regulators. e Increased RNA:DNA hybrid formation resulting from depletion of endogenous ADAR1 was rescued by infection of ADAR1p110-WT (wild type) but not by infection of ADAR1p110-E912A deamination defective mutant or ADAR1p150-WT. c–e Data are mean ± SD (n = 3, biological replicates); significant differences were identified by two-tailed Student’s t tests: *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant. All individual experimental data values and exact P values are presented in Source Data file.