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. 2021 Mar 12;12:1654. doi: 10.1038/s41467-021-21921-x

Fig. 3. Accumulation of R-loops at telomeres in ADAR1-depleted cells.

Fig. 3

a ADAR1 depletion had no effects on already known sites prone to the formation of R-loops. Six sites were examined by qPCR analysis of DRIP products. PCR primers used are listed in Supplementary Data 1. Data are mean ± SD (n = 3, biological replicates); significant differences were identified by two-tailed Student’s t tests: n.s., not significant. b DRIP products were subjected to genomic DNA dot blot hybridization analysis with a probe containing the G-rich-telomere canonical repeat (TTAGGG), or a probe for α-satellite repeat, Alu, or LINE1 consensus sequence (Supplementary Data 1). ADAR1 depletion resulted in increased the formation of RNA:DNA hybrids specifically at telomeric repeats, which was abolished by E. coli-RNase H treatment prior to DRIP. c Significance of the increased R-loop formation at telomeric repeats was confirmed by conducting three independent dot blot hybridization analyses of DRIP products. Data are mean ± SD (n = 3, biological replicates); significant differences were identified by two-tailed Student’s t tests: **P < 0.01, n.s., not significant. a, c All individual experimental data values and exact P values are presented in Source Data file.