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. 2021 Mar 12;12:1654. doi: 10.1038/s41467-021-21921-x

Fig. 4. ADAR1p110 cannot edit completely matched RNA:DNA hybrids carrying telomeric repeat sequences.

Fig. 4

a Canonical telomeric repeat sequences of G-strand RNA (red) and C-strand DNA (blue) are shown. Six adenosines of the G-strand RNA and twelve adenosines of the C-strand DNA are indicated by numbers 1–6 and 1–12, respectively. b In vitro editing assay for completely matched telomeric repeat dsRNA was conducted using ADAR1p110-WT recombinant protein. c In vitro editing assay for completely matched telomeric repeat RNA:DNA hybrids using ADAR1p110-WT recombinant protein. No significant levels of editing for matched RNA:DNA hybrids were detected. b, c PCR products (RT-PCR-amplified RNA strands and PCR-amplified DNA strands) were subjected to Sanger sequencing. The editing frequency was estimated as the % ratio of the guanosine (black) peak over the sum of guanosine and adenosine (green) peaks of the sequencing chromatograms. Editing frequency estimated for three independent experiments (n = 3, technical replicates) is presented in Supplemental information (Supplementary Data 2 and Source Data file).