Fig. 5. Detection of telomeric variant repeats in ALT-positive and non-ALT cancer cell lines.

a Dot blot hybridization analysis for genomic DNA samples was conducted using three separate telomeric repeat probes capable of distinguishing a single-nucleotide mismatch (Supplementary Fig. 4a, b). In addition to canonical TTAGGG signals, varying amounts of TCAGGG and TTGGGG variant repeat signals were detected in both ALT and non-ALT cancer cell lines, but not in primary human fibroblast cells. b Quantitation of canonical and variant telomeric repeats was done by comparing dot blot signals of genomic DNA and canonical and variant repeat-specific control oligos. Three independent dot blot hybridization analyses were performed. Data are mean ± SD (n = 3, biological replicates). All individual experimental data values are presented in Source Data file.