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. 2021 Mar 12;12:1654. doi: 10.1038/s41467-021-21921-x

Fig. 9. ADAR1 regulates accumulation of telomeric R-loops only in non-ALT cells.

Fig. 9

a Detection of increased RNA:DNA hybrids only in non-ALT cells. Genomic DNA samples (0.25 µg) collected from various cells treated with siControl or siADAR1-1 RNAs for 72 h were examined by dot blot assay using the S9.6 antibody. Data are mean ± SD (n = 3, biological replicates); significant differences were identified by two-tailed Student’s t tests: *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant. b DRIP products from select cell lines were further subjected to dot blot analysis for the C-strand telomeric repeat DNA using the canonical telomeric repeat (TTAGGG) specific probe (Supplementary Fig. 4a). Telomeric repeat RNA:DNA hybrids were detected only in HEK293T and HCT116 non-ALT cancer cell lines. Data are mean ± SD (n = 3, biological replicates); significant differences were identified by two-tailed Student’s t tests: *P < 0.05, **P < 0.01, n.s., not significant. a, b All individual experimental data values and exact P values are presented in Source Data file.