Table 3.

Evaluated sequences of anti-kynurenine antibodies. The sequences are categorized into sequences derived from machine learning (ML), sequences derived from NGS total reads (Freq), and the parental sequence (Control). Residues in diversified positions are shown. Likelihood was calculated by the constructed machine learning model. Dissociation constant (K_D) against kynurenine was measured by SPR.

Analysis	Position																	NLL	KD(M)
	28	31	33	50	51	52	54	55	56	58	96	97	99	100	100a	100b	100c
ML	W	R	L	V	V	S	S	G	R	R	A	P	R	G	R	P	H	9.01	5.7E-07
ML	W	R	L	V	V	S	S	G	V	R	V	P	S	G	P	P	H	9.03	1.9E-06
ML	W	R	L	V	V	S	S	G	R	R	V	P	Y	G	R	P	H	9.04	9.6E-08
ML	W	A	L	V	V	S	S	G	S	L	V	P	R	G	P	P	H	9.06	9.9E-08
ML	W	R	L	V	V	N	S	G	R	R	V	P	L	G	R	P	H	9.09	5.6E-08
ML	W	R	L	V	V	N	S	G	S	R	A	P	R	G	L	P	H	9.23	7.2E-07
ML	W	R	L	V	V	N	S	G	S	L	V	P	R	G	S	P	H	9.24	6.6E-08
ML	W	R	L	V	V	D	S	G	V	R	V	P	S	G	R	P	H	9.27	4.2E-08
ML	W	R	L	V	V	S	S	G	S	L	A	P	I	G	T	P	H	9.30	4.6E-07
ML	W	V	L	V	V	N	S	G	S	L	V	P	S	S	P	P	H	9.30	8.7E-08
Freq	S	V	R	T	V	L	L	G	H	D	S	P	H	I	R	P	H	28.00	4.3E-06
Freq	W	N	V	A	I	S	L	S	A	H	S	A	V	M	D	A	H	23.00	3.9E-06
Freq	D	L	T	R	V	T	Q	G	E	V	V	P	R	L	R	P	H	22.00	3.7E-07
Freq	W	L	S	S	V	A	H	S	T	V	A	P	R	P	P	A	R	20.00	4.4E-06
Freq	W	N	T	Y	L	V	S	G	K	M	V	P	I	R	L	P	H	13.00	3.0E-06
Freq	R	W	S	I	V	V	S	G	W	L	V	P	G	G	N	P	H	13.00	9.2E-07
Freq	W	S	S	W	L	G	M	S	A	L	V	A	D	R	S	P	H	13.00	1.9E-06
Freq	N	S	I	A	I	S	A	G	L	Y	V	P	S	G	S	A	H	13.00	2.8E-07
Freq	W	S	Y	S	L	L	H	G	V	G	V	P	D	G	L	P	H	12.00	5.9E-06
Freq	H	H	M	S	V	R	S	G	A	Q	S	P	F	F	R	A	H	12.00	1.5E-05
Control	T	S	A	G	I	I	F	G	T	N	A	P	V	A	R	P	R	-	7.7E-05