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. 2021 Mar 12;12:1645. doi: 10.1038/s41467-021-21938-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a FTH1 levels by immunoblot on whole cell lysates from human CD34+ progenitors cultured 3 days in iron-replete (100% TSAT) or deficient (10% TSAT) erythroid medium ± 1–5 mM isocitrate (IC) and ±1 mM fumarate (Fum or F). Colored boxes highlight synergy of 3 mM IC with 1 mM Fum. Red: iron restriction with no or single metabolite treatment; green: iron restriction with combined metabolite treatment. Representative results from three independent experiments. b Graph: mean normalized FTH1 signal (±3 mM IC and ±1 mM Fum), relative to value with 100% TSAT. Error bars, SEM; n = 3 biologically independent experiments; *, **, ***P = 0.02, 0.009, 0.0009, one-way ANOVA with Tukey post hoc. NS not significant. See also Supplementary Fig. 9. Source data are provided as a Source data file.