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. 2021 Mar 12;11:5838. doi: 10.1038/s41598-021-84708-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EZH2 depletion promotes resistance in the myeloid cell line K562. (a) Comparison of IC₅₀ values for DNR and AraC in twelve haematopoietic cell lines. Cells were treated with AraC/DNR for 72 h. (b) Immunoblot for EZH2 expression and global H3K27me3 of seven EZH2^−/− and six EZH2^wt sc clones in K562 cells. MW, molecular weight; β-actin and H3 total, loading controls. (c) Comparison of AraC IC₅₀ values in EZH2^wt (n = 6) and EZH2^−/− (n = 7) clones. Cells were treated with AraC/DMSO for 72 h. Each value represents the mean of three independent experiments. (d) Proliferation of EZH2^wt (n = 4) and EZH2^−/− (n = 7) clones for 5 d. Medium was changed every 48 h. (e), Long-term low dose AraC treatment in EZH2^wt (n = 3) and EZH2^−/− (n = 3) clones. Cells were treated with 30 nM AraC/DMSO for 12 d. Viable cells relative to untreated control. Unpaired, two-tailed Student’s t-test; *p < 0.05; **p < 0.01; ***p < 0.001. Error bars indicate mean ± s.d of three independent experiments.