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. 2021 Mar 12;11:5834. doi: 10.1038/s41598-021-85142-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Images of a leaflet before and after melanin bleaching in 2.5% H₂O₂ solution. (a) Bright field image of the whole leaflet (red contour) before bleaching showing visible accumulations of melanin (red arrowhead). (b) CARS image of the region of the annulus in the red box in (a) acquired before bleaching with the adipocytes clearly identified by the intense CARS signal of the lipid droplets. (c) SHG image of the region in the red box in (a) acquired before bleaching showing the dense mesh of collagen fibers. (d) Bright field image of the whole leaflet (red contour) after bleaching without any visible melanin. (e) CARS image of the region in the red box in (d) acquired after bleaching with the adipocytes clearly identified by the intense CARS signal of the lipid droplets. (f) SHG image of the region in the red box in (d) showing no alterations of the mesh of collagen fibers after bleaching.