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. 2021 Mar 12;11:5834. doi: 10.1038/s41598-021-85142-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative analysis of the collagen content in a leaflet using SHG images. The mean collagen content from WT mice and ApoE^−/− mice from (a) the region 1; from (b) region 2; and from (c) the region 3. Representative SHG images of the region 1 of the aortic valve leaflets from (d) WT mice and (e) ApoE^−/− mice. Representative SHG images of the region 2 from (f) WT mice and (g) ApoE^−/− mice. Representative SHG images of the region 3 of (h) WT mice and (i) ApoE^−/− mice, acquired in the middle of the thickness. The dimensions of all images are 140 µm × 140 µm.