Fig. 1. Expression and genomic binding of SWI/SNF subunits in adipogenesis.

a Schematic of subunits in SWI/SNF complexes BAF, PBAF and GBAF (ncBAF). b Expression profiles of SWI/SNF subunits in adipogenesis of immortalized brown preadipocytes (n = 1). Published RNA-Seq data sets were used (GSE74189)¹⁸. D-3, day -3. D2, day 2. Expression profiles of SWI/SNF subunits in adipogenesis of 3T3-L1 white preadipocytes and a different brown preadipocyte cell line are presented in Supplementary Fig. 1a. c Genomic distributions of SMARCA4 (BRG1), SS18, BAF-specific ARID1A, PBAF-specific ARID2 and GBAF-specific BRD9 before (D-3) and during (D2) adipogenesis of brown preadipocytes were determined by ChIP-Seq. Promoters were defined as transcription start sites ±1 kb. Active enhancers were defined as H3K4me1⁺ H3K27ac⁺ promoter-distal regions. Primed enhancers were defined as H3K4me1⁺ H3K27ac⁻ promoter-distal regions. Numbers of binding sites are indicated. d Motif analysis of SWI/SNF subunits binding regions at D-3 and D2 of adipogenesis using SeqPos motif tool. Top 3,000 significant binding regions were used. e Venn diagrams depicting genomic binding of SMARCA4, SS18, ARID1A, ARID2 and BRD9 at D2 of adipogenesis. BAF, PBAF, and GBAF binding sites were defined as SMARCA4⁺ SS18⁺ ARID1A⁺, SMARCA4⁺ ARID2⁺, and SMARCA4⁺ BRD9⁺ regions, respectively. f Venn diagram depicting genomic binding of BAF, PBAF and GBAF at D2 of adipogenesis.