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. 2021 Mar 12;12:1630. doi: 10.1038/s41467-021-21893-y

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Smarca4^AID/AID brown preadipocytes were infected with retroviruses expressing C/EBPβ or vector (Vec), and then infected with retroviruses expressing Tir1. Sub-confluent cells were treated with auxin for 4 h and collected for Western blot, ChIP-Seq and ATAC-Seq analyses. a Western blot analyses using antibodies indicated on the left. n = 3 biological replicates. b Among the 449 C/EBPβ-activated enhancers in preadipocytes, 220 exhibited SMARCA4-independent C/EBPβ binding, while the remaining 229 showed SMARCA4-dependent C/EBPβ binding. c Motif analysis of SMARCA4-independent and -dependent C/EBPβ-activated enhancers in preadipocytes using SeqPos motif tool. d, e Auxin-induced, Tir1-dependent acute depletion of SMARCA4 reduces MLL4 binding on C/EBPβ-activated enhancers in preadipocytes. Heat maps (d) and average profiles (e) around C/EBPβ-activated enhancers are shown. Enhancers shown in the heat maps were ranked by the intensity of C/EBPβ at the center in control cells expressing ectopic C/EBPβ. f A model depicting the interdependent relationship between BAF and MLL4 in promoting pioneer TF- and LDTF-dependent cell type-specific enhancer activation.

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