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. 2021 Mar 12;6:35. doi: 10.1038/s41541-021-00300-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Composition of vaccine formulations. Rows detail subtype composition. n/a indicates no strain was present for that subtype. b Ferrets were intranasally vaccinated at Study Day 0 with monovalent, trivalent, or quadrivalent LAIV formulations over a range of doses. Gray arrows indicate major interventions over the 31-day study course. Colored boxes highlight sampling points, with time in days on horizontal axis. Red arrow indicates hourly telemetry recording of core body temperature. *Representative of 2015–16 commercial formulation. d day, A/BOL13 A/Bolivia/559/2013, A/NC99 A/New Caledonia/20/1999, A/SWITZ13 A/Switzerland/9715293/2013, B/BRIS08 B/Brisbane/60/2008, B/PHUK13 B/Phuket/3073/2013, FFU fluorescent focus units, LAIV live attenuated influenza vaccine, NT nasal turbinate.