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. 2021 Mar 12;11:5823. doi: 10.1038/s41598-021-84234-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Rationale behind using density- and size-based EV isolation techniques in tandem to deplete non-EV particles from plasma or serum (modified after²¹). (B) Overview of isolation procedures investigated in this study. Black dots indicate that the preparative step (grey row labels) or analytic step (white row labels) was a task in the procedure.