Table 1.
Donor characteristics and sample details used for miRNA profile characterisation.
| Donor | D1 | D2 | D3 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gender | Female | Female | Male | |||||||||
| Age | 29 | 42 | 28 | |||||||||
| Fasting | No | No | No | |||||||||
| Blood product type | CPRP | hypACT | CPRP | hypACT | CPRP | hypACT | ||||||
| Fraction type | biofluid | EVs | biofluid | EVs | biofluid | EVs | biofluid | EVs | biofluid | EVs | biofluid | EVs |
| Particles/ml blood product | n.d | 1.03E + 09 | n.d | 5.46E + 08 | n.d | 7.86E + 08 | n.d | 8.20E + 08 | n.d | 9.26E + 08 | n.d | 2.98E + 08 |
| Average mode particle size [nm] ± SD | n.d | 179 ± 5 | n.d | 161 ± 26 | n.d | 155 ± 8 | n.d | 167 ± 5 | n.d | 199 ± 27 | n.d | 177 ± 10 |
| Hemolysis indicator ΔCq(miR-23a—miR-451a) [Blondal et al. 2013] | 0.82 | 1.60 | 10.23 | 2.02 | 0.13 | 0.60 | 10.23 | 0.88 | -0.98 | -1.14 | 9.93 | 3.50 |
| Total miRNAs | 332 | 275 | 332 | 215 | 327 | 267 | 324 | 200 | 336 | 285 | 310 | 184 |
| Relevant miRNAs (Cq < 35) | 275 | 149 | 254 | 49 | 282 | 150 | 251 | 64 | 273 | 185 | 227 | 39 |
| Exluded miRNAs (Cq ≥ 35) | 57 | 126 | 78 | 166 | 45 | 117 | 73 | 136 | 63 | 100 | 83 | 145 |
| Undetected miRNAs | 40 | 97 | 40 | 157 | 45 | 105 | 48 | 172 | 36 | 87 | 62 | 188 |
| Estimated total miRNA copy number | 4.37E + 10 | 6.27E + 07 | 1.02E + 11 | 3.64E + 06 | 4.46E + 10 | 9.58E + 07 | 9.05E + 10 | 5.00E + 06 | 2.21E + 10 | 2.26E + 08 | 1.85E + 10 | 1.68E + 06 |
| Proportion of EVs carrying at least 1 miRNA | n.d | 2.44% | n.d | 0.27% | n.d | 4.88% | n.d | 0.24% | n.d | 9.77% | n.d | 0.23% |
| 1 out of n EVs bears any miRNA | n.d | 41 | n.d | 370 | n.d | 20 | n.d | 385 | n.d | 10 | n.d | 382 |
| One single copy of a miRNA species per n particles, on average ± SD | n.d | 2.59E + 04 ± 2.85E + 04 | n.d | 2.20E + 04 ± 1.64E + 04 | n.d | 1.75E + 04 ± 2.13E + 04 | n.d | 2.36E + 04 ± 2.03E + 04 | n.d | 1.30E + 04 ± 1.98E + 04 | n.d | 1.47E + 04 ± 8.62E + 03 |
Concentrations and mode sizes of EVs were determined in SEC fractions via NTA before final pooling via UC.
n.d. not determined.