Fig. 4. Incorporating aptardi transcripts into the original transcriptome improves the ratio of true positive to false positive 3′ termini compared with the original transcriptome and compared with the Tool for Alternative Polyadenylation site AnalysiS (TAPAS) analysis on the original transcriptome.

Results from transcripts added by aptardi to the original transcriptome are shaded in dark. Transcripts whose 3′ terminus was plus or minus 100 bases of a true polyadenylation site from PolyA-Seq data were considered a true positive and otherwise counted as a false positive. Data shown are from the Human Brain Reference data set.