REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E. coli DH10B ElectroMax	Thermo Fisher Scientific	Cat#18290015
Chemicals, Peptides, and Recombinant Proteins
Lipofectamine 2000 Transfection Reagent	Invitrogen	Cat#11668019
Schneider’s Modified Medium	Lonza	Cat#04-351Q
Critical Commercial Assays
PureLink RNA Micro Kit	Invitrogen	Cat#12183016
ActinGreen 488 ReadyProbes Reagent	Thermofisher Scientific	Cat#R37110
LunaScript RT SuperMix Kit	New England Biolabs	Cat#E3010S
Luna Universal qPCR Master Mix	New England Biolabs	Cat#M3003S
DNeasy Blood & Tissue Kit	QIAGEN	Cat#69504
MEGAscript RNAi T7 Kit	Life Technologies	Cat#AM1626
One-Step RT-PCR System	Invitrogen	Cat#12574026
Deposited Data
RNA-Seq data	This study	ENA: PRJEB38605
Experimental Models: Cell Lines
A. gambiae Sua5.1	60	N/A
Experimental Models: Organisms/Strains
A. gambiae G3 strain	BEI Resources	MRA-112
A. stephensi AF-SDA500 strain	Infravec2	V.2.3.1.L.FR.1.0
Oligonucleotides
See Table S2 for detailed information on used oligonucleotides	This study	N/A
Recombinant DNA
p165	61	GenBank: KU189142
pUAST-attB	62	GenBank: EF362409
pBac_pattPs_fle_3miR	This study	GenBank: MW147152
pENTR R4-vas2-Transposase-R3	63	N/A
pGEM-T Easy Vector System	Promega	Cat#A1360
Software and Algorithms
BLAST	64	https://blast.ncbi.nlm.nih.gov/Blast.cgi
Jpred-4	65	http://www.compbio.dundee.ac.uk/jpred/
ClustalX2	66	https://clustalx.software.informer.com/2.1/
MEGA v. 7.0.26	67	https://www.megasoftware.net/
WebLogo	68	https://weblogo.berkeley.edu/logo.cgi
ImageJ	69	https://imagej.nih.gov/ij/
Kallisto v0.46	70	https://pachterlab.github.io/kallisto/
DESeq2	71	http://bioconductor.org/packages/release/bioc/html/DESeq2.html
apeGLM	72	https://bioconductor.org/packages/release/bioc/html/apeglm.html
Other
QuantStudio 3 Real-Time PCR System	Applied Biosystems	Cat#A28136