Figure 3. Ly6G+SigF^HI granulocytes are neutrophils, not eosinophils.

A, Flow cytometry data of leukocytes isolated from ΔdblGATA mice on D4‐post MI and immunostained for Cd11b, Ly6G, and SiglecF (% of Cd11b cells; mean±SD). B, Quantification of classically defined neutrophils, eosinophils, and Ly6G+SiglecF^HI granulocytes. C, Heatmap showing relative gene expression of canonical eosinophil genes in SigF^HI and SigF^LO neutrophils and eosinophils (Han et al, 2018 ³⁰ ). D, Spearman rank correlation analysis showing similarity of SigF^HI and SigF^LO neutrophils of present study to previously published lung cancer model. ¹¹ MI indicates myocardial infarction; scRNA, single‐cell RNA; SigF, siglecf; SigF^HI, siglecf high; and WT, wild‐type. *P<0.05, **P<0.01, Student's t test.