Skip to main content
. 2021 Jan 20;10(3):e017633. doi: 10.1161/JAHA.120.017633

Figure 5. CCN2 (cellular communication network factor 2) deficiency reduces the development of abdominal aortic aneurysm in elastase‐induced mouse model of abdominal aortic aneurysm.

Figure 5

(A), Representative aortae from the elastase‐induced experimental mice at day 14 showing maximal aortic width in the infra‐renal region. Scale bar, 1 mm. (B), Quantification of external diameter of infrarenal aortae (n=3 for controls, n=5 for Ccn2+/+ elastase and Ccn2+/‐ elastase). (C), Representative images of infrarenal aorta stained with hematoxylin and eosin, trichrome, and Verhoeff‐Van Gieson staining illustrating the extent of abdominal aortic aneurysm progression, fibrosis and elastin fragmentation, respectively, at day 14 in the experimental mice. (D), Representative immunohistochemistry images showing expression of active caspase‐3, matrix metalloproteinase 2, and smoothelin‐B in the aortic tissues at day 14 in experimental mice (n=5). (E), Quantification of collagen, elastin, active caspase‐3, matrix metalloproteinase 2, and smoothelin B in the aortic tissues of experimental mice (n=4). Scale bar=50 µm. ***P<0.001, ns=non‐significant in Tukey multiple comparisons test.