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. 2020 Dec 21;10(1):e018993. doi: 10.1161/JAHA.120.018993

Figure 3. Colchicine inhibits NETosis in primed neutrophils from patients with ACS independently of ROS generation.

Figure 3

Neutrophils from patients with ACS and healthy participants were isolated and (A) NETosis imaged after 2 hours in unstimulated or PMA‐stimulated cells. NET release was measured using a fluorescent plate–based method in (B) unstimulated cells or in cells stimulated with (C) PMA or (D) ionomycin. Neutrophils from patients with ACS were isolated and treated with 25 nmol/L colchicine for 1.5 hours before measurement of NET release in (E) unstimulated cells or in cells stimulated with (F) PMA or (G) ionomycin. Neutrophils from patients with ACS were isolated and treated with 25 nmol/L colchicine for 1.5 hours before quantification of reactive oxygen species production using dihydrorhodamine 123 in (H) unstimulated, (I) PMA‐, and (J) ionomycin‐stimulated cells. Error bars represent SD from the mean. Scale bar=20 µm. ***P<0.001; **P<0.01; *P<0.05. ACS indicates acute coronary syndrome; AUC, area under the curve; MFI, median fluorescence intensity; PMA, phorbol 12‐myristate 13‐acetate; and NETs, neutrophil extracellular trap.