Table 2. Amino acid identity and cross reactivity to H5N1 NP peptides.
| Epitope/ subtype | Sequence | Cross reactivity | Frequency of recognition |
|---|---|---|---|
| NP1-20 | |||
| H5 | MASQGTKRSYEQMETGGERQ | – | 1* survivor |
| H3 | ***************D*D** | ||
| H1 | ***************D**** | ||
| H1pdm | ******************** | ||
| NP111-130 | |||
| H5 | YDKEEIRRIWRQANNGEDAT | + | 5 (1* survivor) |
| H3 | ******************** | ||
| H1 | ****************D*** | ||
| H1pdm | *****V************** | ||
| NP221-240 | |||
| H5 | RMCNILKGKFQTAAQRAMMD | + | 6 |
| H3 | ******************** | ||
| H1 | ***************K**** | ||
| H1pdm | ******************V* | ||
| NP311-330 | |||
| H5 | QVFSLIRPNENPAHKSQLVW | + | 3 |
| H3 | *IY***************** | ||
| H1 | *VY***************** | ||
| H1pdm | *****M************** | ||
| NP401-420 | |||
| H5 | ASAGQISVQPTFSVQRNLPF | + | 2 |
| H3 | *****T************** | ||
| H1 | *******T************ | ||
| H1pdm | ******************** | ||
| NP411-430 | |||
| H5 | TFSVQRNLPFERATIMAAFT | – | 1* survivor |
| H3 | ***********KS******* | ||
| H1 | **********DKT******* | ||
| H1pdm | *******************S | ||
| NP481-498 | |||
| H5 | MNNEGSYFFGDNAEEYDN | + | 3 |
| H3 | *S**************** | ||
| H1 | *S**************** | ||
| H1pdm | *S***************S |
Notes.
*
H5N1 survivor subject.
NP from H5N1 strain A/Thailand/1(KAN-1)/2004 (H5N1) (accession no. AAV35112); H3N2 strain A/Brisbane/10/2007 (H3N2) (accession no. ACO95274); H1N1 strain A/New Caledonia/20/1999 (H1N1) (accession no. ABW81621); H1N1pdm strain A/Thailand/104/2009 (H1N1) (accession no. ACR23306).
- Cross reactivity: (-) epitope recognized by H5N1 survivor only; (+) epitope recognition by H5N1 survivor and/or non-H5N1 subjects.