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. 2021 Mar 9;14:1807–1819. doi: 10.2147/OTT.S286820

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© 2021 Yue et al.

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Circ-PITX1 silencing inhibited NSCLC progression in vitro. HCC827 and H1650 cells were transfected with si-NC or si-circ-PITX1. (A) The expression of circ-PITX1 was detected by qRT-PCR. CCK8 assay (B), colony formation assay (C), flow cytometry (D), wound healing assay (E) and transwell assay (F) were used to measure the viability, colony number, apoptosis, migration and invasion of cells. (G and H) The cell cycle process was analyzed using flow cytometry. (I) H1650 cells were transfected with sh-NC or sh-circ-PITX1. The expression of circ-PITX1 was measured by qRT-PCR to confirm the transfection efficiency of sh-circ-PITX1. (J–L) H1650 cells transfected with sh-NC or sh-circ-PITX1 were injected into nude mice. (J) Tumor volume was measured in each group. (K) After 32 days, the tumors were removed and photographed. (L) Tumor weight was detected in each group were measured. *P < 0.05, **P < 0.01, ***P < 0.001.